Coding

Part:BBa_K2641000:Design

Designed by: Selina Beck   Group: iGEM18_FAU_Erlangen   (2018-08-24)


CDS of S-layer protein PS2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1387
    Illegal XhoI site found at 214
    Illegal XhoI site found at 832
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 202
    Illegal AgeI site found at 1369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1366
    Illegal SapI site found at 613
    Illegal SapI site found at 825


Design Notes

silent mutation of a PstI sequence for BioBrick compatibility (24T>A) silent mutations for lower complexity (621G>A; 624T>A; 747C>A)


Source

Synthetic gene. BioBrick compatible variant of cspB gene of Corynebacterium glutamicum ATCC 14067.

References